Figure 2. Effects of cold-inducible RNA-binding protein (CIRBP) on cell apoptosis of H9C2 cells with myocardial ischemia. Cells were transfected with pcDNA3.1, pc-CIRBP, siNC or siCIRBP and cultured in serum-free medium with hypoxia. Cell apoptosis was evaluated by flow cytometry. pc-CIRBP: pcDNA3.1 containing CIRBP coding sequence; siCIRBP: CIRBP-specific small interfering RNA; siNC: siCIRBP negative control. Data are reported as means±SD of 3 independent experiments. *P<0.05, pc-CIRPB compared to pcDNA3.1 and siCIRBP compared to siNC (ANOVA).