Figure 3. Effects of cold-inducible RNA-binding protein (CIRBP) on reactive oxygen species (ROS) levels of H9C2 cells with myocardial ischemia. Cells were transfected with pcDNA3.1, pc-CIRBP, siNC or siCIRBP and cultured in serum-free medium with hypoxia. The ROS level was evaluated by flow cytometry using DCFH-DA. pc-CIRBP: pcDNA3.1 containing CIRBP coding sequence; siCIRBP: CIRBP-specific small interfering RNA; siNC: siCIRBP negative control; DCFH-DA: 2,7-dichlorofluorescein diacetate. Data are reported as means±SD of 3 independent experiments. *P<0.05, siCIRBP compared to siNC; ***P<0.001, pc-CIRPB compared to pcDNA3.1 (ANOVA).
