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. 2017 Apr 22;6:e24845. doi: 10.7554/eLife.24845

Figure 7. Synaptic stimulation does not increase SV mobility.

Analysis of C5E-Syt2-labeled SVs in giant calyceal terminals. (A) KCl stimulation: Scatter plot of SV trajectory lengths and maximum speeds superimposed with individual trajectory traces in control terminals (Red) or terminals incubated with 65 mM KCl (Blue). (B) Sucrose stimulation: Scatter plot of SV trajectory lengths and maximum speeds superimposed with individual trajectory traces in control terminals (Red) or terminals incubated with 500 mM sucrose (Blue). (C) Electrical simulation: Scatter plot of SV trajectory lengths and maximum speeds superimposed with individual trajectory traces in control terminals (Red) or terminals during 1 Hz electrical field stimulation for 30 s (Blue). (D) Trajectory length analysis in control (Red) and KCl-treated terminals, sucrose-treated terminals, or 1 Hz-stimulated (Blue) terminals. (E) Displacement modality analysis in control (Red) and KCl-treated terminals, sucrose-treated terminals, or 1 Hz-stimulated (Blue) terminals. (F) Diffusion coefficient analysis in control (Red) and KCl-treated terminals, sucrose-treated terminals, or 1 Hz-stimulated (Blue) terminals. (KCl treatment: n = 6; sucrose treatment: n = 6; 1 Hz stimulation: n = 6 in D, (E and F). Two-tailed unpaired t-test (*p<0.05; ns, not significant).

DOI: http://dx.doi.org/10.7554/eLife.24845.035

Figure 7—source data 1. Data and statistics for Figure 7D, E and F.
DOI: http://dx.doi.org/10.7554/eLife.24845.036
elife-24845-fig7-data1.xlsx (13.8KB, xlsx)
DOI: 10.7554/eLife.24845.036
Figure 7—source data 2. Data and statistics for Figure 7—figure supplement 1C.
DOI: http://dx.doi.org/10.7554/eLife.24845.037
elife-24845-fig7-data2.xlsx (8.8KB, xlsx)
DOI: 10.7554/eLife.24845.037

Figure 7.

Figure 7—figure supplement 1. SV mobility does not change after spontaneous or stimulated uptake.

Figure 7—figure supplement 1.

(A) Upper panels: confocal images of C5E-Syt2 labeled vesicles loaded during spontaneous activity for 1 hr or during train of 1 Hz electrical stimulation. Lower panel: Number (N) and fluorescence intensity (FI) of C5E-Syt2-labeled vesicles loaded spontaneously or during 1 Hz stimulation (Blue bar). (B) Scatter plot of SV trajectory lengths and maximum speeds superimposed with individual trajectory traces during spontaneous (Red) or stimulated activity (Blue). (C) Diffusion coefficients of C5E-Syt2-labeled vesicles loaded during spontaneous (Red, n = 3 terminals) or stimulated (Blue, n = 3) activity. Two-tailed unpaired t-test (ns, not significant).
DOI: http://dx.doi.org/10.7554/eLife.24845.038