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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Biochem J. 2016 Nov 30;474(3):357–376. doi: 10.1042/BCJ20160760

Figure 7. Inserting the α-subunit TMD1 or TMD2 into βENaC restores Lhs1-dependent degradation of αENaC.

Figure 7

Cycloheximide chase reactions were performed as described in the Experimental Procedures section in WT (filled circles) and Δlhs1 (open circles) yeast strains transformed with plasmids designed to express αENaC-HA, γENaC-V5 and (A) βENaCαTM1-13myc or (B) βENaCαTM2-13myc. Lysates were prepared and resolved proteins were immunoblotted with anti-HA antisera (αENaC) and with anti-G6P as a loading control. The expression of myc-tagged and γENaC proteins were confirmed by western blot (data not shown). Glycosylated (+g) and unglycosylated (−g) species are indicated. Data represent the means of 8–11 experiments, ±SEM. *P < 0.05, **P < 0.01, ***P < 0.001.