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a) Illustration of the v-SNARE / t-SNARE vesicle fusion process monitored by formation of the Cy3-CB[7]•Ad-Cy5 FRET affinity pair, and b) fluorescence intensity time traces for three individual fusion events with different levels of multistep flickering as reported by the Cy3-CB[7]•Ad-Cy5 FRET affinity pair. (Reprinted by permission from ref 37. Copyright 2015, American Chemical Society).
