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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: New Phytol. 2017 Mar 13;214(4):1673–1687. doi: 10.1111/nph.14517

Fig. 3.

Fig. 3

Several defense responses are enhanced in Pseudomonas syringae pv. tomato (Pst) DC3000-resistant Arabidopsis accessions. (a) Pathogenesis-related gene 1 (PR1) protein accumulation 24 h after H2O and 100 μM benzothiadiazole (BTH) treatment in select Pst DC3000-resistant accessions shows an enhanced response after elicitation with BTH. Two samples are shown per each treatment. Untreated (-) Col-0 is shown as a negative control. Proteins were detected using α-PR1 antibodies while the asterisk points to the expected molecular weight of PR1. Bottom image shows the Ponceau S staining of the polyvinylidene difluoride (PVDF) membranes. Twenty μg of total protein were loaded per well. (b) Reactive oxygen species (ROS) production in Col-0 and resistant accessions in response to 2 × 108 colony-forming units (CFU) ml−1 Pst DC3000 or 0.25 mM MgCl2 as the ROS elicitors. RLU, for relative light units. Error bars show the 95% confidence intervals for the means. Detection was done using SpectraMax L microplate reader (Molecular Devices).