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. 2017 May 10;7:171. doi: 10.3389/fcimb.2017.00171

Figure 1.

Figure 1

Construction of the fluorescently labeled EGFP-EV71 virus and its phenotype analysis. (A) Construction of the fluorescently labeled EGFP-EV71 virus. The green line represents the EGFP fragment and the red line represents the fragment encoding the AITTLGS peptide, the latter of which is capable of being recognized as a cleavage site by the viral-encoded 2A enzyme. (B) EGFP-EV71 and WT-EV71 growth curves in Vero cells. The data are presented as the mean ± SD of three independent replicates. (C) The level of EGFP-EV71 neutralization by an anti-EV71 antibody. The fluorescence intensities of EGFP were detected with exciting light: 485/20 nm and emission light: 528/20 nm. The significance of each difference was determined using an unpaired Student's t-test, two-tailed; **p < 0.01, and ***p < 0.001. (D) The plaques formed by EGFP-EV71 and WT-EV71 in Vero cells were stained using crystal violet and scanned to locate the fluorescence as described in the Materials and Methods Section.