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. 2017 May 10;7:171. doi: 10.3389/fcimb.2017.00171

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Copyright © 2017 Zhao, Zhang, Zhang, Feng, Fan, Wang, Liu, Wang, Wang, Zhang, Wang, Liao, He, Lu, Yang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Assessment of EGFP-EV71 infection of cultured DCs. (A) Flow cytometry was performed to detect the distribution of DC subsets infected by EGFP-EV71 in vitro. The data are presented as the mean ± SD, n = 3; t-test, two-tailed; ^*p < 0.05, ^**p < 0.01. (B) Viral titration analysis of EGFP-EV71-infected DCs. The data are presented as the mean ± SD, n = 3. (C) Cultured DCs were infected by EGFP-EV71, and their green fluorescence expression was assessed by fluorescence microscopy. (D–E) In vitro EGFP-EV71-infected CD141⁺ DCs (red) were labeled with an anti-CD141 antibody (D) and anti-CLEC9A antibody (E). (F) Cytokines detected in the supernatant of EGFP-EV71-infected cultured DCs.