Table 1.

Primer sequences.

Primer	Sequence(5′ to 3′)	Fragment
EV71-HindIII-1F	TACAAGCTTTTAAAACAGCCTGTGGGTTGCAC	EV71-A
EV71-754R	TTGCTCACCGAACCCATGTTTAGCTGTGT
EGFP-1F	CATGGGTTCGGTGAGCAAGGGCGAG	EGFP-1
EGFP-1R	AGGGTAGTAATGGCCTTGTACAGCTCGTCCAT
EGFP-2F	TAACACAGCTAAACATGGGTTCGGTGAGCAAG	EGFP-2
EGFP-2R	CACTTGTGAGCCAAGGGTAGTAATGGCCTT
EV71-745F	CCCTTGGCTCACAAGTGTCTACACAGCG	EV71-B
EV71-SalI-3711R	CCAGTCGACACTATGCCGACGACGC
EV71-HindIII-1F	TAC(AAGCTT)TTAAAACAGCCTGTGGGTTGCAC	EV71-C
EV71-X-754R	CAAGGCCATTACTACCCTTGGCTCACAAGTG

The primer sequences marked with underlined Letters represent the nucleotide-encoding peptide (AITTLGS) cleavage recognition site recognized by the viral-encoded 2A enzyme.