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. 2017 May 10;8:263. doi: 10.3389/fphar.2017.00263

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Copyright © 2017 Caballero, Aira, Lavastida, Popa, Rivero, González, Mesa, González, Coba, Lorenzo-Luaces, Wilkinson, Santiesteban, Santiesteban, Troche, Suarez, Crombet, Sánchez, Casacó, Macías and Mazorra.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Binding of pre-immune and hyperimmune sera of vaccinated patients to the A431 cells. Recognition assay was performed by incubating the 1:10 diluted sera with A431 cells at different time points, in the pre-immune (PI), in days 56, 112, and 168, as well as 28 days after ending treatment (Day 196). (A) Mean of post-immune recognition after baseline values subtraction obtained for all patients in every dose. Recognition of A431 cells (>20%) was seen in patients received higher doses of vaccine. (B) Histogram of A431 recognition by sera from one representative patient belonging to 800 μg is shown.