Figure 1.

Focused areas of brain sections for immunostaining. Five (or four for P6 and three for P3-to-5-day-old mice) 100-μm-thick sections were prepared at intervals of 400 μm from one brain. The measures indicate the distances from the first section in which the rostral tip of the hippocampus appeared. Sections were immunolabeled for aggrecan and RGS14 and counterstained with blue-fluorescent Nissl. Regions, including the CA2 area and dentate gyrus (DG), indicated by boxes (A) and (B), respectively, were confocally imaged and Z-stacked.