Figure 6.

Homer 1a influences mGluR5-extracellular signal-regulated kinase (ERK) signaling. Mice cortical neuronal cultures were pretreated with 2-methyl-6-(phenylethynyl)pyridine (MPEP) (5 μM). After traumatic injury, phosphorylation of ERK was measured by western blot analysis (a). The data are represented as the mean±S.E.M. from five experiments. (RS)-2-chloro-5-hydroxyphenylglycine (CHPG) (500 μM) was administered in normal neuronal cultures or neuronal cultures subjected to traumatic injury. ERK phosphorylation was assessed by western blot analysis (b and c). The data are represented as the mean±S.E.M. from five experiments. *P<0.05 versus vehicle. Neuronal cultures were pretreated with CHPG (500 μM), PD98059 (40 μM), or U0126 (20 μM). Cell viability (d), cytotoxicity (e), and cell death rate (f) were measured in neuronal cultures after traumatic injury. The data are represented as the mean±S.E.M. from five experiments. *P<0.05 versus vehicle. Mice cortical neuronal cultures were transfected with different lentiviruses. Phosphorylation of ERK was measured by western blot analysis after CHPG (500 μM) treatment (g). The data are represented as the mean±S.E.M. from five experiments. *P<0.05 versus vector; ^#P<0.05 versus shRNA Con. After transfection with different lentiviruses, neuronal cultures were pretreated with CHPG (500 μM) and Ro 67-7476 (10 μM). Phosphorylation of ERK was measured by western blot analysis after traumatic injury (h)