Figure 7.

Homer 1a affects mGluR1-extracellular signal-regulated kinase (ERK) signaling by regulating intracellular Ca²⁺. Mice cortical neuronal cultures were pretreated with 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA-AM) (10 μM). Cell viability, cytotoxicity, and cell death rate were measured in neuronal cultures after traumatic injury (a). The data are represented as the mean±S.E.M. from five experiments. *P<0.05 versus vehicle. Neuronal cultures were pre-treated with Bay 36-7620 (10 μM). Intracellular Ca²⁺ concentrations were measured until the indicated times (b). The data are represented as the mean±S.E.M. from five experiments. *P<0.05 versus vehicle. Neuronal cultures were pretreated with BAPTA-AM. After treatment with Ro 67-7476 (10 μM), phosphorylation of ERK was measured by western blot analysis (c). The data are represented as the mean±S.E.M. from five experiments. *P<0.05 versus vehicle. Mice cortical neuronal cultures were transfected with different lentiviruses. After transfection, the intracellular Ca²⁺ concentrations were measured until the indicated times after traumatic injury (d and e). The data are represented as the mean±S.E.M. from five experiments. *P<0.05 versus vector; ^#P<0.05 versus short hairpin RNA (shRNA) Con. Mice cortical neuronal cultures were transfected with different lentiviruses. After pretreatment with Bay 36-7620 (10 μM), the intracellular Ca²⁺ concentrations were measured until the indicated times in neuronal cultures subjected to traumatic injury (f and g). The data are represented as the mean±S.E.M. from five experiments. Mice cortical neuronal cultures were transfected with different lentiviruses. After transfection, the intracellular Ca²⁺ concentrations were measured until the indicated times in neuronal cultures treated with Ro 67-7476 (10 μM) (h and i). The data are represented as the mean±S.E.M. from five experiments. *P<0.05 versus vector; ^#P<0.05 versus shRNA Con