Figure 8.

Homer 1a influences mGluR5-ERK signaling by mediating protein kinase C (PKC) activity. Mice cortical neuronal cultures were pretreated with chelerythrine chloride (CTC) (10 μM) or Bryostatin 1 (5 μM). Cell viability, cytotoxicity, and cell death rate were measured in neuronal cultures after traumatic injury (a). The data are represented as the mean±S.E.M. from five experiments. *P<0.05 versus vehicle. Neuronal cultures were pretreated with (RS)-2-chloro-5-hydroxyphenylglycine (CHPG) (500 μM) and CTC (10 μM), and phosphorylation of ERK was measured by western blot analysis (b). The data are represented as the mean±S.E.M. from five experiments. *P<0.05 versus vehicle. PKC activity was assessed in neuronal cultures treated with CHPG (500 μM) (c). The data are represented as the mean±S.E.M. from five experiments. *P<0.05 versus vehicle. Mice cortical neuronal cultures were transfected with different lentiviruses. After transfection, the neuronal cultures were treated with CHPG (500 μM). The activity of PKC was measured in neuronal cultures subjected to traumatic injury (d) or normal neuronal cultures (e). The data are represented as the mean±S.E.M. from five experiments. *P<0.05 versus vector; ^#P<0.05 versus shRNA Con. After Bryostatin 1 (5 μM) treatment, PKC activity was assessed in neuronal cultures transfected with different lentiviruses (f). The data are represented as the mean±S.E.M. from five experiments