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. 2014 Apr 10;5(4):e1157. doi: 10.1038/cddis.2014.135

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Copyright © 2014 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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LKB1 locates to centrosomes and mitotic spindle poles. Proliferating U2OS cells were fixed and immunostained with monoclonal antibody to LKB1 and polyclonal antibody to γ-tubulin (a and b) or α-tubulin (c). The white arrows show the colocalization of the two proteins. (d) U2OS cells were transfected with GFP-LKB1. At 24 h hours after transfection, cells were fixed and stained with anti-pericentrin antibody. The white arrow shows the colocalization of LKB1 and pericentrin. The insets show the enlarged location of GFP-LKB1 in the centrosome