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. 2017 May 8;8:15318. doi: 10.1038/ncomms15318

Figure 1. Schematic of imaging genomic loci with quantum dot-labelled TALEs.

Figure 1

Within the cytosol of a live cell, the TALEs fused with a short LplA acceptor peptide (LAP) is decorated with trans-cyclooctene and subsequently labelled with tetrazine-conjugated red QD (QD625) via Diels–Alder cycloaddition chemistry. The TALE fused with an AP tag is biotinylated and labelled with streptavidin-conjugated green QDs (QD525). The two QD-TALEs bind to the target HIV-1 proviral DNA sequences, and their fluorescence co-localization demonstrates single-copy HIV-1 provirus loci in human chromosomes.