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. 2017 May 8;8:15318. doi: 10.1038/ncomms15318

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Schematic of HIV-1 proviral genome illustrating the distribution of U3, R and U5 regions in 5′-LTR. (b) Schema depicting the pair of N2 TALEs and their binding sites on the HIV-1 proviral genome. The constructed TALEs contain the central DNA-binding domain with 19.5 and 16.5 repeat units (green boxes), respectively, and nuclear localization sequence at the N-terminus. The target sequences are highlighted in red. (c) Activity of six custom TALE pairs targeting diverse sequences of LTR in a reporter-based yeast assay. Activity was measured in a yeast-based assay in which cleavage and recombination reconstitutes a functional lacZ gene. Activity was normalized to a PC positive control, and NC indicated mock control. Error bars denote s.d.; n=3.