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. 2017 May 8;8:15318. doi: 10.1038/ncomms15318

Figure 4. Labeling TALEs with QD625 via the Diels–Alder cycloaddition in live cells.

Figure 4

(a) Confocal images showing TALE N2-L labelled with QD625 in live cells. (b) Confocal images showing Tz1-QD625 transfected into a live U1 cell. (c) Diagram of the TALE-GFP-fusion protein. (d) Confocal images showing TALE-GFP transiently expressed in living cells (scale bar, 5 μm). (e) Percentage of QD625s (2,000 QD625s in 125 cells) with or without attachment to TALE re-localized to the nucleus. Inset: count and statistics of QD625s with or without attachment to TALE in the cytoplasm and nucleus. Error bars denote s.d.; n=125.