Figure 5. Deletion of the Prkca gene restores mitochondrial function.
(a) Defective mitochondria (Mito) rates and total number of mitochondria in embryonic sections. Defective mitochondria rate=number of defective mitochondria divided by total number of mitochondria per image area (image size: 9.43 μm2) in neuroepithelial cells based on the images of defective and normal mitochondria in supplementary fig. 2. Neuroepithelia from three embryos (N=3) derived from different dams were used. Three serial sections per embryo were analysed. The effect of Prkca gene deletion on protein abundance of Bak (b), Bax (c), Puma (d) and Bim (e) in isolated mitochondria of E8.75 embryos. Bar graphs for protein abundance were quantitative data from three independent experiments. *indicates significant difference compared with other groups in one-way ANOVA followed by Tukey tests. P<0.05.