Figure 3.
Effects of CQCQD and carbachol on mRNA expression of PLC-β1, PKC isoforms, and AC in colonic SMCs. (a) Representative images of agarose gel electrophoresis of targeted RT-PCR mRNAs. (b) PLC-β1 mRNA, (c) PKC-α, (d) PKC-β, (e) PKC-ε, and (f) AC. Relative expression of individual mRNA is calculated according to β-actin. ∗P < 0.05 versus control group; †P < 0.05 versus L-arginine group. Values are means ± SEM of 5–11 animals per group.