TABLE 1 .
Haplotypes of pfcrt-modified and parental Dd2 parasites
| Parasite linea | Altered PfCRT haplotype | Donor plasmid | ZFN plasmid | PfCRT haplotype at positionb: |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 72 | 74 | 75 | 76 | 101 | 220 | 271 | 326 | 356 | 371 | ||||
| Dd2 | No | None | None | C | I | E | T | C | S | E | S | T | I |
| Dd2Dd2 | No | pcrtDd2-hdhfr | pZFN14/15-bsd | C | I | E | T | C | S | E | S | T | I |
| Dd2Dd2+C101F CL1 | Yes | pcrtDd2+C101F-hdhfr | pZFN14/15-bsd | C | I | E | T | F | S | E | S | T | I |
| Dd2Dd2+C101F CL2 | Yes | pcrtDd2+C101F-hdhfr | pZFN14/15-bsd | C | I | E | T | F | S | E | S | T | I |
| 3D7 | No | None | None | C | M | N | K | C | A | Q | N | I | R |
a
The name of the transfected strain is shown, followed by a superscript, which refers to the transfected haplotype. 3D7 was added to show the canonical wild-type haplotype observed in chloroquine-sensitive parasites.
b
Underlined letters indicate residues that were modified compared to the parental line.