TABLE 3.
Primers used for validation of RNA-seq data using the Fluidigm Biomark HD high-throughput qPCR system
| Associated gene name | Sequence |
Ensembl transcript identifier | |
|---|---|---|---|
| Forward primer | Reverse primer | ||
| ACKR2 | GACATCCAGCTCTCAGAGACA | CACGTGTTGGTGATGCTCAA | ENSGALT00000008506 |
| AJAP1 | GCCTGGAGATTACAAAGCAACC | AGACACAAAGGCCACAGGAA | ENSGALT00000044613 |
| APOA1 | CCCTCGCTGTGCTCTTCC | GCCTTCACCGTCTCCAGGTA | ENSGALT00000011524 |
| CASP7a | ATGACCGAAGCTGTGAGGATA | GGCAAAACAAGCAGCATCAC | ENSGALT00000014519 |
| CASP9a | TTTCAGGTCCCTGTGCTTCC | TTCCGCAGCTCCACATCAA | ENSGALT00000002085 |
| CCR8 | TCTGTGAGGCTTTCGGCAAA | TCCGTGTTGCGTCTGTTGAA | ENSGALT00000019499 |
| CD4 | AGTGGAACCTGGATGTGTCA | TTTCCAAGCGTTCCTTCTCAAA | ENSGALT00000037104 |
| CD40a | AGCCTGGTGATGCTGTGAA | CTCACAGGGTGTGCAGACA | ENSGALT00000039105 |
| CMPK2 | AGGCTGAACTGGAAGCTAACA | CTTGGCACGCAGGATTCAC | ENSGALT00000043751 |
| COL2A1_s5 | GGTCCTTCCGGCTTCCA | CAGGAACACCCTGGTCTCC | ENSGALT00000035835 |
| COL5A2 | TGGGAACCACCTGACACAAA | GAGCCAAACGTCAGCTTCAA | ENSGALT00000042826 |
| COL9A2 | CCAAGGCTTGCCAGGAGTCA | CCTTTGGGACCGGTCTTTCC | ENSGALT00000043674 |
| CREB3L1 | ATGCTGAAACCAGCGAGGTA | AACAGCTGCCTGCTCACTTA | ENSGALT00000013673 |
| CRISP3 | AGGCGAGACTTCCAATCTTCC | GACAGCACAGCTGCAAGAC | ENSGALT00000026918 |
| H6PDa | ATGTACCGGGTGGACCACTA | AACTGACGGTTCTGATCTCGAAA | ENSGALT00000003926 |
| HCK | ACTGGAAACAGAGCTCAGAGAC | TCTGCAGGAAGCAGAGAAACA | ENSGALT00000010538 |
| IL-18a | CGTGGCAGCTTTTGAAGATGTA | CTGAATGCAACAGGCATCCC | ENSGALT00000012787 |
| IL1Ba | TGCTTCGTGCTGGAGTCAC | GGCATCTGCCCAGTTCCA | ENSGALT00000000738 |
| IL-6a | AACGTCGAGTCTCTGTGCTA | AGTCTGGGATGACCACTTCA | ENSGALT00000017759 |
| IL-8a | CCCCACTGCAAGAATGTTGAAA | GTGCCTTTACGATCAGCTGTAC | ENSGALT00000042745 |
| ITK | TGGAACAAGTGCCAGACCAA | AAGTCGCTATGGCTTTGGCTA | ENSGALT00000006130 |
| LAG3 | CAGAACCAGAGCAGCAGAAAC | CAACAGTGACAGCACAGCAA | ENSGALT00000023369 |
| LITAFa | ATCGTGACACGTCTCTGCTA | AGCATCAACGCAAAAGGGAA | ENSGALT00000005095 |
| MAPK8IP3a | GCCAAAGCCAAAATGGAAACC | GTCGAGCAACAATCGCTTCA | ENSGALT00000002721 |
| MHCI-likeY | GCCGGAACGCTACAACAAA | TCCAGGATGTCACAGCCAAA | ENSGALT00000009998 |
| MMP7 | CGGGACAGGCAGACATCA | TGTGCCACCTCTTCCATCAA | ENSGALT00000027770 |
| MOV10 | CAAGAAGCTGTTGAAGCCCTAC | AGGAAAGTTGGCCGATACCA | ENSGALT00000002368 |
| Mx | CTGTTGCGATGCTGAACAAA | TTAGCAAAACGCTTGTTAGCC | ENSGALT00000025999 |
| MYO7A | TGAGGCAGGAATCCTTAGGAAC | ACTGCCACCAGTATTGAACCA | ENSGALT00000001046 |
| NLRC5a | TTTGCTGCTGCGCTTTCA | TGTGATGCTTCCACCTGTCA | ENSGALT00000001908 |
| PRKCB | ATGTGGTACAGATGCCAACCA | AGCCCCAAACAGAACGGTAA | ENSGALT00000009676 |
| RHPN2 | TGACCGCACATACCGTAACA | AAGAGCTGTCCTGAACCAACA | ENSGALT00000007686 |
| RPL4a | TTCTGCCTTGGCAGCATCA | AGGAAGTTCTGGGATCTCCTCA | ENSGALT00000012495 |
| SDHAa | CGTGATCTGGCTCATCTAAAGAC | TTGCAGTTCAAGGGTCTCCA | ENSGALT00000021508 |
| SERPINB2a | AAATCACCGTGCCTGTTTCC | GTGCTTTAAACCCAGCATGGATA | ENSGALT00000020982 |
| SLC16A6 | TCACAGCAGTGGCATCTACA | CAGCCAATCTGCTCCTTCAAA | ENSGALT00000006617 |
| SPON2 | TCACACCCAAGGTGGGAAAA | ACTCTGTGCTCCCTGCAAA | ENSGALT00000045656 |
| TGFB3a | GGGCCCTGGATACCAACTAC | GGTCCTGTCGGAAGTCAATGTA | ENSGALT00000037707 |
| TLR4a | CCTGCTGGCAGGATGCA | TGTTCTGTCCTGTGCATCTGAA | ENSGALT00000011333 |
| TRAF6a | TGCCCCAGTACCATGCTTTTA | GTGTCGTGCCAGTTCATTCC | ENSGALT00000012888 |
| TRAT1 | CGTAGCACAGCACAGAAACA | ATTGTCCCCTGCTTCACTCA | ENSGALT00000036882 |
| WNT4a | AGCAAAGGGGCATCTTCCAA | TCCACCCGCATGTTGTTCA | ENSGALT00000007645 |
| Worthington | TTCACAGGCACCTACATCACA | GGTGTTTTGTGTCCCATGCA | ENSGALT00000003855 |
| ZNFX1 | TGCTGAAGTCTGCTGCTGTA | AACATTGGCCGTTCACTGAC | ENSGALT00000007763 |
a
The primers were used/analyzed previously (62).