FIG 5.

Lipoprotein substrate competition assay. Extracts from lpp null strains harboring various combinations of lipoprotein N-acylating enzymes (+ or −) and Lpp(K58A)–Strep-tagged substrate were separated by SDS-PAGE and immunoblotted with anti-Strep-tag antibody–HRP conjugate. Strain KA528 was grown with (lane 1) and without (lane 2) l-arabinose, while strains KA532 (lane 3), KA583 (lane 4), KA548 (lane 5), KA599 (lane 6), KA600 (lane 7), and TXM327 (lane 8) were grown in LB medium. Parentheses indicate that lnt expression was controlled by adding l-arabinose to the growth medium.