FIG 7.

MALDI-TOF MS-MS of E. faecalis PnrA and B. cereus PrsA. (A to C) MS-MS spectra of the m/z 997 parent ions corresponding to the N-terminal lipopeptides of E. faecalis PnrA purified from the wild-type strain (A), the deletion strain (B), and the plasmid back-complemented strain (C). (F to H) MS-MS spectra of the m/z 1334 parent ion corresponding to the N-terminal lipopeptide of B. cereus PrsA purified from the wild-type strain (F), the deletion strain (G), and the back-complemented strain (H). (D, E, I, J) The elucidated lyso- and diacylglycerol structures are shown for PnrA (D and E) and PrsA (I and J). Images of the SDS-PAGE gel and the parent ion spectra can be found in Fig. S2 and S3, respectively, in the supplemental material.