TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid(s) | Relevant genotype/phenotypea | Source |
|---|---|---|
| E. coli strainsb | ||
| BW25113 | E. coli K-12 wild type; Δ(araD araB)567 ΔlacZ4787(::rrnB-3) λ− rph-1 Δ(rhaD-rhaB)568 hsdR514 | CGSC7636c |
| TXM315 | gut::Kanr-rrnB TT-araC-PBAD-lnt | This study |
| TXM327 | lpp::Chlr | This study |
| KA325 | ybeX-(Kanr-rrnB TT-araC-PBAD)-lnt | This study |
| KA349 | TXM327 ybeX-(Kanr-rrnB TT-araC-PBAD)-lnt | This study |
| KA472 | TXM315 lnt::Sptr | This study |
| KA486 | TXM327(pEF4) | This study |
| KA489 | TXM327(pBC7) | This study |
| KA494 | TXM327(pUC19) | This study |
| KA495 | TXM327(pUC19-lnt) | This study |
| KA503 | pUC19 | This study |
| KA504 | pUC19-lnt | This study |
| KA505 | pEF4 | This study |
| KA528 | KA349(pKA524) | This study |
| KA532 | KA486 with lnt::Sptr by transduction and pKA522 | This study |
| TXM537 | KA325(pEF4) | This study |
| TXM538 | KA325(pUC19) | This study |
| TXM539 | KA349(pEF4) | This study |
| TXM540 | KA349(pUC19) | This study |
| TXM541 | KA472 chiQ::Aprr | This study |
| KA548 | TXM327(pKA522) | This study |
| KA555 | pBC7 | This study |
| KA583 | KA489 with lnt::Sptr by transduction and pKA522 | This study |
| KA597 | KA325(pBC7) | This study |
| KA598 | KA349(pBC7) | This study |
| KA599 | TXM327(pEF4, pKA522) | This study |
| KA600 | TXM327(pBC7, pKA522) | This study |
| Firmicutes strains | ||
| TXM465 | E. faecalis ATCC 19433 | Sigma |
| KA477 | B. cereus ATCC 14579 | American Type Culture Collection |
| KA543 | TXM465 ΔWMC_RS08810 | This study |
| KA546 | KA477 ΔBC1526 | This study |
| KA637 | KA546(pKA637) | This study |
| KA666 | KA543(pKA635) | This study |
| Plasmids | ||
| pUC19, pCL25, and pKFC | General cloning vectors; Carr | |
| pEF4 | pUC19 bearing E. faecalis genomic DNA; Carr | This study |
| pBC7 | pUC19 bearing B. cereus genomic DNA; Carr | This study |
| pKA495 | pUC19-lnt; Carr | This study |
| pKA522 | lpp(K58A)-Strep; Kanr | This study |
| pKA524 | lpp(K58A)-Strep; Sptr | This study |
| pKA635 | pMSP3535-WMC_RS08810; Eryr | This study |
| pKA637 | pSP-BC1526-hp; Tetr | This study |
a
Resistance phenotypes: Carr, carbenicillin; Kanr, kanamycin; Sptr, spectinomycin; Chlr, chloramphenicol; Eryr, erythromycin; Tetr, tetracycline; Aprr, apramycin.
b
All E. coli strains are derivatives of BW25113.
c
Strain CGSC7636 at the Coli Genetic Stock Center (CGSC).