TABLE 1.
Quantification of mKate2 expression of the different reporter strainsa
| Reporter strain | Ciprofloxacin treatment | AM | GM | % DAGb | Fold induction upon treatmentc |
|---|---|---|---|---|---|
| E. coli PrecA-gfp | − | 2.9 | 2.6 | 11.5 | 28.6 |
| + | 82.8 | 75.4 | 9.8 | ||
| A. baumannii | |||||
| PrecA-No UTR-mKate2 | − | 8.8 | 8.4 | 4.8 | 1.3 |
| + | 11 | 10.5 | 4.8 | ||
| PrecA-UTR(C::G)-mKate2 | − | 10.7 | 8.8 | 21.6 | 2.5 |
| + | 27.1 | 14.9 | 81.9 | ||
| PrecA-UTR(ΔOL)-mKate2 | − | 38.7 | 31.6 | 22.5 | 4.2 |
| + | 163.2 | 132.4 | 23.3 | ||
| PrecA-UTR(G::G)-mKate2 | − | 6.6 | 6.2 | 6.5 | 2.3 |
| + | 15.2 | 13.6 | 11.8 | ||
| PtrpB-No UTR-mKate2 | − | 6.3 | 6.3 | 0 | 1 |
| + | 6.5 | 6.5 | 0.62 | ||
| PtrpB-UTR(C::G)-mKate2 | − | 39.1 | 33.1 | 18 | 1.01 |
| + | 39.4 | 32.8 | 20.4 |
a
The fluorescence expression of >1,000 cells of each A. baumannii strain was quantified using the MicrobeJ plug-in for ImageJ (37). The arithmetic mean (AM) and geometric mean (GM) for each population were calculated.
b
The percent difference between the arithmetic mean and the geometric mean (DAG) was determined as 100 × (AM − GM)/AM.
c
Fold induction of mKate2 expression upon treatment with 10× MIC of Cip was determined as AMTreated/AMUntreated.