Fig. 4.

Knockdown of CASP8 or NGFR increases the invasiveness of GBM cells.

(A, C) LN229 cells were transfected with CASP8 (A) or NGFR (C) siRNAs. After 48 h, the level of CASP8 or NGFR was examined by western blot analysis, and invasiveness was assessed by Transwell invasion assay. Invaded cells were photographed under the microscope and counted from more than twelve fields. (B, D) T98G cells were transfected and invasive activity of transfected cells was assessed as described above. All experiments were performed more than three times and data are represented as mean ± S.D.