Fig. 6.

Defects in the brain and retina of ranbp9-MO injected zebrafish embryos.

(A) Control (Cont) and ranbp9-MO injected (MO) zebrafish larvae at 5 dpf. Compared to normal development of body trunk region, reduced size of brain and eye was observed in the MO injected larva. Asterisk indicates swim-bladder. (B) By acridine orange staining, a specific cell death was detected in the brain tectum of 54 hpf morphants. (C) Disruption of retinal development in ranbp9 morphant. Section of HE-stained retina in control and ranbp9 morphant at 73 hpf. In wild-type, the retina was established into several layers; 1, ganglion cell layer: 2, inner plexiform layer: 3, inner nuclear layer: 4, outer plexiform layer: 5, outer nuclear layer: and 6, photoreceptor cell layer, respectively. In morphants, however, these layer formations were not well established.