Table 1.
Gr. | Sub-group | No. | Reducing agent | Activity [%] MtLPMO9Ba | Activity [%] MtLPMO9B + AbPPOb | Activity [%] MtLPMO9B + MtPPO7b |
---|---|---|---|---|---|---|
I | a | 1 | 4-Hydroxybenzoic acid | 100 | 119 | 90 |
2 | Ortho-cresol | 100 | 778 | 2 | ||
3 | Para-coumaric acid | 100 | 9938 | 2851 | ||
4 | Phenol | 0c | >0 c | 0c | ||
I | b | 5 | 3-Hydroxy-4-methoxycinnamic acid | 100 | 151 | 7558 |
6 | 4-Hydroxy-3-methoxyphenylacetone | 100 | 117 | 131 | ||
7 | Coniferyl aldehyde | 100 | 128 | 562 | ||
8 | Ferulic acid | 100 | 107 | 231 | ||
9 | Guaiacol | 100 | 461 | 5143 | ||
10 | Hesperidin | 100 | 56 | 771 | ||
11 | Homovanillic acid | 100 | 156 | 580 | ||
12 | Vanillic acid | 100 | 196 | 495 | ||
I | c | 13 | 4-Allyl-2,4-methoxyphenol | 100 | 89 | 108 |
14 | Sinapic acid | 100 | 110 | 195 | ||
15 | Syringic acid | 100 | 107 | 142 | ||
II | a | 16 | 3,4-Dihydroxybenzoic acid | 100 | 99 | 139 |
17 | 3-Methylcatechol | 100 | 58 | 104 | ||
18 | caffeic acid | 100 | 55 | 122 | ||
II | b | 19 | 3,4-Dihydroxy-5-methoxybenzoic acid | 100 | 99 | 22 |
20 | 3,4-Dihydroxy-5-methoxycinnamic acid | 100 | 99 | 116 | ||
III | 21 | Gallic acid | 100 | 105 | 124 |
Division based on functional groups of reducing agents (Fig. 2)
aTotal release of non-oxidized and C1-oxidized gluco-oligosaccharides from RAC incubated with MtLPMO9B set to 100%
bIncreased (italics ≥50% increase) or decreased percentage of released non-oxidized and C1-oxidized gluco-oligosaccharides from RAC incubated with MtLPMO9B with addition of either AbPPO or MtPPO7 compared to the release of non-oxidized and C1-oxidized gluco-oligosaccharides from RAC incubated with MtLPMO9B alone. Sum of areas of released non-oxidized and C1-oxidized gluco-oligosaccharides are shown in Fig. 2. See “Methods” for more information
cNo reference due to absent activity of MtLPMO9B towards RAC in the presence of phenol (Fig. 2)