Table 2. Analysis by RT-qPCR of nitrate-induced genes in the wild-type, nasT, and nasS mutant strains of P. denitrificans.

Data correspond to the mean ± standard deviation of the glutamate plus nitrate/glutamate gene expression ratio.

Gene		Wild-type Glu + NO3−/Glu	nasT mutant Glu + NO3−/Glu	nasS mutant Glu + NO3−/Glu
Pden_0490	gltD	0.6 ± 0.1	0.7 ± 0.1	2.6 ± 0.2
Pden_0794	bztB	2.2 ± 1.6	0.7 ± 0.4	–
Pden_1212	ureD	1.1 ± 0.4	1.0 ± 0.1	1.8 ± 0.1
Pden_2032	amtB	0.4 ± 0.0	0.6 ± 0.1	0.3 ± 0.1
Pden_2151	potD	0.5 ± 0.1	0.6 ± 0.1	–
Pden_4019	urtC	0.5 ± 0. 1	0.5 ± 0.1	–
Pden_4129	ntrC	0.4 ± 0.1	0.5 ± 0.1	0.8 ± 0.2
Pden_4130	ntrB	0.7 ± 0.1	0.6 ± 0.2	0.6 ± 0.1
Pden_4234	narJ	7.7 ± 1.2	57.0 ± 14.4	–
Pden_4236	narG	2.5 ± 0.2	67.5 ± 12.2	173.8 ± 12.3
Pden_4450	nasH	0.6 ± 0.2	0.9 ± 0.1	0.2 ± 0.1
Pden_4453	nasA	0.9 ± 0.3	0.3 ± 0.1	0.2 ± 0.1
Pden_4454	nasS	1.0 ± 0.3	1.4 ± 0.1	0.0 ± 0.0
Pden_4455	nasT	2.0 ± 0.4	0.0 ± 0.0	1.8 ± 0.1
Pden_4461	glnB	2.0 ± 0.2	0.4 ± 0.1	1.0 ± 0.1
Pden_4462	glnA	1.7 ± 0.1	0.4 ± 0.1	1.2 ± 0.1
Pden_3872	gdhA	2.0 ± 0.3	1.1 ± 0.2	11.4 ± 1.0