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. 2017 May 10;474(11):1769–1787. doi: 10.1042/BCJ20170115

Table 3. Analysis of the nasT promoter by lacZ (β-galactosidase activity) gene fusion in P. denitrificans wild-type and ntrB and ntrY mutant strains.

Strain/construct N-source
NH4+ NO3 Glu Glu + NO3
WT/PnasT (native) 71 ± 5 14 500 ± 143 11 000 ± 120 15 000 ± 152
WT/PnasT (mutated)* 50 ± 3 220 ± 14 53 ± 5 600 ± 25
ntrY/PnasT (native) n.d. n.d. 11 500 ± 115 15 200 ± 135
ntrB/PnasT (native) n.d. n.d. 10 ± 1 120 ± 4
*

The base substitutions are specified in Supplementary Table S2. Abbreviations: n.d., not determined.