Table 3. Analysis of the nasT promoter by lacZ (β-galactosidase activity) gene fusion in P. denitrificans wild-type and ntrB and ntrY mutant strains.
Strain/construct | N-source | |||
---|---|---|---|---|
NH4+ | NO3− | Glu | Glu + NO3− | |
WT/PnasT (native) | 71 ± 5 | 14 500 ± 143 | 11 000 ± 120 | 15 000 ± 152 |
WT/PnasT (mutated)* | 50 ± 3 | 220 ± 14 | 53 ± 5 | 600 ± 25 |
ntrY/PnasT (native) | n.d. | n.d. | 11 500 ± 115 | 15 200 ± 135 |
ntrB/PnasT (native) | n.d. | n.d. | 10 ± 1 | 120 ± 4 |
The base substitutions are specified in Supplementary Table S2. Abbreviations: n.d., not determined.