Figure 1.

Design of Artificial miRNAs Targeting Human Huntingtin

(A) Position of target sites on the human huntingtin mRNA. Target sites are distributed throughout the coding region. The location of the qPCR assay, which spans the junction between exons 64 and 65, is also depicted. (B) AAV9 constructs expressing a miRNA from the CβA (polII) and U6 promoters. The CβA promoter-driven miRNA is located in the 3′-UTR of the GFP gene (top), whereas the construct containing the U6 promoter-driven artificial miRNA co-expresses GFP from a separate promoter (bottom).