Figure 5.

NPNT (nephronectin) is proadhesive in normal adrenal and aldosterone-producing adenoma (APA) cells, but antiadhesive in H295R. A, NPNT is proadhesive in (i) HEK, (ii) normal primary adrenal, (iii) zona fasciculata (ZF)–like APA, and (iv) zona glomerulosa (ZG)–like APA, but antiadhesive in (v) H295R, as demonstrated by wells precoated with PBS, BSA, NPNT, or laminin, and measured by Xcelligence cell impedance as cell index over time (4 h for cell lines, 10 h for primary adrenal cells; n=2 for ZF-like APA and ZG-like APA, n=4 for the rest). B, NPNT is antiadhesive in H295R cells, as confirmed by Hoechst fluorescent stain assay measuring % maximum fluorescence as a representation of number of cells adhered to well with increasing concentrations of BSA, NPNT, and laminin precoating (n=3 for each protein at each concentration). Bars represent mean values per group±SEM.