FIGURE 1.

(A) Diagram of measurement locations for both the pCASL labeling experiments (blue slice, located 2 cm distal from labeling plane) and perfusion quantification experiments (purple slice, located 6 cm distal from labeling plane). For both experiments, the location of the pCASL labeling plane was approximately at the level of the popliteal artery (gray slice). For PASL, sliceselective and nonselective inversion was used for label and control conditions, respectively, and the perfusion measurement slice remained at the same location as in pCASL (purple slice). (B) Data to estimate stability of pCASL efficiency were acquired from repeated ischemia-reperfusion paradigms in which the imaging readout immediately followed the control or label period, and were normalized by an image in which no labeling was conducted (reference acquisition-shown in the top line of B). A fourth scan was conducted to measure blood flow in the popliteal artery during the ischemia-reperfusion paradigm at the level of the labeling plane. (C) Timing diagram for PASL and pCASL perfusion quantification sequences. C and L refer to control (nonselective inversion) and label (slice-selective inversion) conditions, respectively for the PASL scan.