Figure 1.
Glutamatergic innervation of thalamic trigeminovascular neurons. (A) Left: Immunopositive VGluT2 synaptic vesicles (green) surrounding a thalamic dura-sensitive neuron (red) labeled with TMR–dextran. Arrowheads indicate close apposition of VGluT2 positive axons and the cell body and dendrites of the labeled neuron. Upper right: Location of the dura-sensitive neuron (red star) shown at left. Number in red indicates distance from bregma (mm). Lower right: Fluorescent images showing VGluT2 axonal labeling in thalamic Po and VPM nuclei. Scale bars = 100 mm. (B) Close apposition between VGluT2 immunopositive vesicles and thalamic trigeminovascular neurons. The three views in the x-y, y-z and x-z planes provide evidence that VGluT2 immunopositive vesicles (green) may contact cell bodies, proximal and distal dendrites of trigeminovascular neurons in LP (red). Arrowheads indicate probable contact point on each view. Note that some green-labeled vesicles and red-labeled soma or dendrites are in the same focal plane (yellow). Scale bar = 50 μm. Adapted from Noseda et al., 201427