. 2017 Feb 8;6(3):121–128. doi: 10.1530/EC-16-0115
This work is licensed under a Table 1.
Primer sequences for real-time RT-PCR.
| Primers | Sequence (5′→3′) |
|---|---|
| Pdx-1 promoter | Forward: GGTACCGGTACCTCCAGTATCAGG |
| Reverse: CTCGAGGAGCTACAAGCCAGGCCT | |
| Pdx-1 real-time PCR | Forward: TAGGCGTCGCACAAGAAGAA |
| Reverse: TCCGTATTGGAACGCTCAAGT | |
| Hepcidin expression | Forward: AAGCTTATGCCTTAGACTGCACA |
| Reverse: ATGAAGACGATTTTATTTTCAGAATTC | |
| Hepcidin real-time PCR | Forward: AAGCAGGGCAGACATTGCGAT |
| Reverse: CAGGATGTGGCTCTAGGCTATGT | |
| Insulin 1 real-time PCR | Forward: CACTTCCTACCCCTGCTGG |
| Reverse: ACCACAAAGATGCTGTTTGACA | |
| Insulin 2 real-time PCR | Forward: GCTTCTTCTACACACCCATGTC |
| Reverse: AGCACTGATCTACAATGCCAC | |
| β-Actin real-time PCR | Forward: CAAGGCCAACCGTGAAAAGAT |
| Reverse: AATGCCAGTGGTACGACCAGAG |