Figure 1. Examples of CSDs obtained by nano-ESI-MS under non-denaturing conditions. The spectra were recorded on a hybrid, quadrupole time-of-flight mass spectrometer (QSTAR Elite, AB-Sciex). Samples were infused at room temperature, by metal-coated borosilicate capillaries with emitter tips of 1 μm internal diameter (Proxeon). The following instrumental settings were applied: declustering potential 60/80 V, ion spray voltage 1.1/1.2 kV, curtain-gas pressure 20 psi. (A) 12 μM β-lactoglobulin in 10 mM ammonium acetate, pH 7.0.¹⁰⁰ (B) 12 μM α-synuclein in 10 mM ammonium acetate, pH 7.4 (negative-ion mode).¹⁰¹ (C) 15 μM Sic1 in 50 mM ammonium acetate, pH 6.5.¹⁰² (D) 10 μM Sic1^215–284 in 50 mM ammonium acetate, pH 6.5.¹⁰³