Figure 5.¹H-¹⁵N HSQC spectra of Tß4 titrated with its partners. (A) ¹H-¹⁵N HSQC overlaps of free (blue) ¹⁵N-Tß4 and with PINCH LIM4–5 (3:1 Tß4 excess, red). (B) ¹H-¹⁵N HSQC overlaps of free (blue) ¹⁵N-tβ4 and with ILK-Ank-GST (3:1 Tß4 excess, blue). (C) ¹H-¹⁵N HSQC overlaps of free (blue) ¹⁵N-Tß4 and with 1:1 mixture of PINCH LIM4–5 and ILK-Ank-GST (3:1 Tß4 excess, red). (D) ¹H-¹⁵N HSQC overlaps of free (blue) ¹⁵N-Tß4 and stabilin CTD (2:1 Tß4 excess, red) in 50 mM sodium acetate and 50 mM NaCl pH 6.5 at 283 K.