Table 2. Summary of the methods used for the characterization of Tß4 binding to its partners.

	BLI	Steady-state fluorescence	Near UV CD	Far UV CD	ITC	NMR	SAXS	Wide-line NMR
Tß4/PINCH LIM4–5	KD = 630 µM	Weak interaction	Interaction with local changes	No structural changes	Too low heat of reaction	Interaction without gaining structure	No major structural changes	Interaction with some buried surfaces
Tß4/ILK-Ank-GST	KD = 380 µM	Weak interaction	Interaction with local changes	No structural changes	Too low heat of reaction	Interaction without gaining structure	No major structural changes	Interaction with some buried surfaces
Tß4/PINCH LIM4–5-ILK-Ank-GST	No information	No information	Interaction with local changes	No structural changes	Too low heat of reaction	Interaction without gaining structure	No information	No information
Tß4/Stabilin CTD	KD = 2.8 mM	No information	Interaction with local changes	No structural changes	Small enthalpy and entropy changes	Interaction without gaining structure	Interaction without gaining structure	Interaction with some buried surfaces