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. 2017 Jan 13;17(1):fox006. doi: 10.1093/femsyr/fox006

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© FEMS 2017.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Relative expression of IMA1-5 in S. cerevisiae strains evolved for faster growth on SM with sucrose as the sole carbon source. Relative expression of IMA5(A); IMA1(B); and IMA2, IMA3 and IMA4(C). Expression of IMA2, IMA3 and IMA4 was assayed by a single primer pair since these genes are highly similar in nucleotide sequence. CEN.PK113-7D: reference strain (IMAx MALxx MPHx SUC2); IMU048: unevolved parental strain (genotype: malΔ mphΔ suc2Δ MAL11); and three independently evolved strains (IMS422, IMA423 and IMS424). CEN.PK113-7D was used as calibrator sample (expression set to 1). Averages and standard errors were obtained from independent duplicate biological experiments and three technical replicates. Cells were harvested at mid-exponential phase (OD_660nm = 3) from shake-flask cultures on SM with sucrose as sole carbon source.