Table 1.
Strains used in this study. The abbreviation malΔ indicates mal11-mal12::loxP mal21-mal22::loxP mal31-32::loxP and mphΔ indicates mph2/3::loxP mph2/3::loxP-hphNT1-loxP.
| Name | Relevant genotype | Parental strain | Origin |
|---|---|---|---|
| CEN.PK113-7D | MATa MAL1x MAL2x MAL3x MAL4x MAL2-8C SUC2 LEU2 URA3 | P. Kötter, Germany | |
| CEN.PK102-3A | MATa MAL1x MAL2x MAL3x MAL4x MAL2-8C SUC2 leu2-112 ura3-52 | P. Kötter, Germany | |
| IMK291 | CEN.PK102-3A MATa leu2-112 ura3-52 MAL2-8C | IMK289 | This study |
| mal11-mal12::loxP mal21-mal22::loxP mal31-32::loxP mph2/3::loxP | |||
| mph2/3::loxP-hphNT1-loxP suc2::loxP-kanMX-loxP | |||
| IMX469 | malΔ mphΔ suc2Δ MAL11-LEU2 | IMK291 | This study |
| IMU048 | malΔ mphΔ suc2Δ MAL11-LEU2 URA3 | IMX469 | This study |
| IMU055 | malΔ mphΔ suc2Δ MAL11-LEU2 MAL12-URA3 | IMX469 | This study |
| IMX470 | malΔ mphΔ suc2Δ LEU2 | IMK291 | This study |
| IMU051 | malΔ mphΔ suc2Δ LEU2 URA3 | IMX470 | This study |
| IMU054 | malΔ mphΔ suc2Δ LEU2 MAL12-URA3 | IMX470 | This study |
| IMZ571 | malΔ mphΔ suc2Δ MAL11-LEU2 cas9-URA3 | IMX469 | This study |
| IMK700 | malΔ mphΔ suc2Δ MAL11-LEU2 cas9-URA3 ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ amdSYM | IMZ571 | This study |
| IMS422 | malΔ mphΔ suc2Δ MAL11-LEU2 URA3 (single colony isolate from evolution, replicate 1) | IMU048 | This study |
| IMS423 | malΔ mphΔ suc2Δ MAL11-LEU2 URA3 (single colony isolate from evolution, replicate 2) | IMU048 | This study |
| IMS424 | malΔ mphΔ suc2Δ MAL11-LEU2 URA3 (single colony isolate from evolution, replicate 3) | IMU048 | This study |
| IMS517 | malΔ mphΔ suc2Δ MAL11-LEU2 | IMS422 | This study |
| IMS518 | malΔ mphΔ suc2Δ MAL11-LEU2 | IMS423 | This study |
| IMS519 | malΔ mphΔ suc2Δ MAL11-LEU2 | IMS424 | This study |
| IMS604 | malΔ mphΔ suc2Δ MAL11-LEU2 cas9-URA3 | IMS517 | This study |
| IMS605 | malΔ mphΔ suc2Δ MAL11-LEU2 cas9-URA3 | IMS518 | This study |
| IMS606 | malΔ mphΔ suc2Δ MAL11-LEU2 cas9-URA3 | IMS519 | This study |
| IMK716 | malΔ mphΔ suc2Δ MAL11-LEU2 cas9-URA3 ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ | IMZ604 | This study |
| IMK717 | malΔ mphΔ suc2Δ MAL11-LEU2 cas9-URA3 ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ | IMZ605 | This study |
| IMK718 | malΔ mphΔ suc2Δ MAL11-LEU2 cas9-URA3 ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ | IMZ606 | This study |
| IMK743 | malΔ mphΔ suc2Δ MAL11-LEU2 ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ amdSYM | IMK700 | This study |
| IMZ620 | malΔ mphΔ suc2Δ MAL11-LEU2 ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ amdSYM IMA1-URA3 | IMK743 | This study |
| IMZ621 | malΔ mphΔ suc2Δ MAL11-LEU2 ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ amdSYM IMA2-URA3 | IMK743 | This study |
| IMZ622 | malΔ mphΔ suc2Δ MAL11-LEU2 ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ amdSYM IMA3,4-URA3 | IMK743 | This study |
| IMZ623 | malΔ mphΔ suc2Δ MAL11-LEU2 ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ amdSYM IMA5-URA3 | IMK743 | This study |