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. Author manuscript; available in PMC: 2017 May 10.
Published in final edited form as: Lab Chip. 2015 Sep 24;15(21):4138–4147. doi: 10.1039/c5lc01000d

Fig. 1.

Fig. 1

Architecture and concept of the integrated micropump in a microfluidic loop of hanging drops. (a) Top view of the design of the hanging-drop network. The pump consists of three drops; seven other drops can be used to culture spheroids. (b) The protocol of the applied pressure for pump actuation is indicated as a square wave and consists of an OFF and ON state, each with a defined duration. The ON state can vary in pressure. Photographs show the open and closed states of the integrated valve. (c) Cross-section AA′ (indicated in Fig. 1a) schematically illustrates the sequence of pump actuation (see text for details); BB′ the drops for spheroid culturing.