Table 2. Characteristics of sequence datasets used for development of new SSR markers.
Sequence dataset | Total number of reads | Number of good quality reads (%) | PALsa | Number of tested loci | Number of loci successfully amplified (%) |
---|---|---|---|---|---|
Enriched Genomic—454 | 556,018 | 18,218 (3.27%) | 270 | 96 | 30 (31.2%) |
Genomic—Illumina | 12,000,000 | 923,229 (7.69%) | 2,720 | 110 | 37 (33.6%) |
EST—454 | 1,317,778 | 393,302 (29.85%) | 397 | 173 | 41 (23.7%) |
a Potentially Amplifiable Loci (PALs).