Table 5.
Results for the 5 candidated miRNA selected for validation. Relative quantification (RQ) calculated with RT-qPCR data as well as p-value are shown for both the discovery and validation cohort. For the discovery cohort, microarray results are also shown for comparison purposes.
| discovery cohort | validation cohort | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| ProbeSet Name | miRNA | R-SW | last/first (SW) | FC (elder/young) LIMMA | adj.P.Val LIMMA | RQ (Qpcr) | p-value | RQ (Qpcr) | p-value |
| hsa-miR-1281_st | miR-1281 | 0.921 | 2.778 | 2.509 | 4.64E-03 | 1.127 | 6.60E-01 | - | - |
| hsa-let-7i_st | let-7i | −0.853 | 0.498 | 0.455 | 4.64E-03 | 0.566 | 2.86E-02 | 0.226 | 1.53E-07 |
| hsa-miR-30b_st | miR-30b | −0.839 | 0.476 | 0.503 | 1.05E-01 | 0.777 | 1.07E-01 | 0.419 | 7.66E-07 |
| hsa-miR-15a_st | miR-15a | −0.874 | 0.465 | 0.525 | 9.39E-02 | 0.632 | 1.21E-01 | 0.219 | 1.23E-07 |
| hsa-let-7g_st | let-7g | −0.899 | 0.434 | 0.468 | 1.54E-02 | 0.578 | 1.14E-02 | 0.199 | 1.05E-08 |