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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: J Immunol. 2017 Mar 17;198(9):3436–3447. doi: 10.4049/jimmunol.1601716

Figure 1. Def6 deficiency enhances macrophage responsiveness to RANKL.

Figure 1

(A) BMMs derived from wild-type (WT) control and Def6−/− mice were stimulated with RANKL at the indicated doses for 4 days. TRAP staining was performed (A) and the number (B, left panel) as well as the area (B, right panel) of TRAP-positive MNCs relative to per well was determined as described in the “Materials and Methods”. TRAP-positive cells appear red in the photographs. Scale bar, 100μm. Data are representative of at least five independent experiments. *P<0.05; **P<0.01. (C) Quantitative real-time PCR analysis of mRNA expression of Acp5 (encoding TRAP), Ctsk (encoding cathepsin K) and Itgb3 (encoding β3) in BMMs from the WT control and Def6−/− mice treated with RANKL (40 ng/ml) for the indicated times. Data are representative of at least three independent experiments. **P<0.01.