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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: J Immunol. 2017 Mar 17;198(9):3436–3447. doi: 10.4049/jimmunol.1601716

Figure 8. IFN-β levels regulate the Def6 effects on osteoclastogenesis and osteoclast gene expression.

Figure 8

(A, C) Osteoclast differentiation induced by TNF-α (40 ng/ml) or RANKL (40 ng/ml) for 3 days in the absence or presence of a serial doses of mouse recombinant IFN-β in the WT control and Def6−/− BMMs. (B, D) Immunoblot analysis of the expression of NFATc1, Blimp1 and c-Fos induced by TNF-α (40 ng/ml) or RANKL (40 ng/ml) without or with IFN-β in the WT control and Def6−/− BMMs. (E, F) BMMs were treated with the control IgG (10 U/ml) or IFN-β neutralizing antibody (10 U/ml) in the presence of RANKL (40 ng/ml) or TNF-α (40 ng/ml) for 4 days. Upper panel: TRAP staining; Lower panel: quantification of the number of TRAP+ MNCs (≥3 nuclei/cell) per well relative to the control.