Table 1. Glycosyl composition of type B, B2, and rough Burkholderia LPS.
B. pseudomallei 576a Type B LPS | B. thailandensis 82172 Type B2 LPS | B. pseudomallei MSHR435 Type R LPS | ||||
---|---|---|---|---|---|---|
Glycosyl residue | Mass (μg) | Mol % | Mass (μg) | Mol % | Mass (μg) | Mol % |
Ribose (Rib) | n.d. | - | 4.6 | 2.7 | n.d. | - |
Arabinose (Ara) | n.d. | - | n.d. | - | n.d. | - |
Rhamnose (Rha) | 81.3 | 43.4 | 93.8 | 50.9 | 1.5 | 3.5 |
Fucose (Fuc) | n.d. | - | n.d. | - | n.d. | - |
Xylose (Xyl) | 17.8 | 10.4 | n.d. | - | n.d. | - |
Glucuronic Acid (GlcA) | n.d. | - | n.d. | - | n.d. | - |
Galacturonic acid (GalA) | n.d. | - | n.d. | - | n.d. | - |
Mannose (Man) | n.d. | - | 1.1 | 0.5 | 1.1 | 2.4 |
Galactose (Gal) | 27.2 | 13.2 | 57.0 | 28.2 | 1.5 | 3.3 |
Glucose (Glc) | 11.3 | 5.5 | 8.0 | 3.9 | 10.9 | 24.0 |
N-Acetyl Galactosamine (GalNAc) | n.d. | - | n.d. | - | n.d. | - |
N-Acetyl Glucosamine (GlcNAc) | n.d. | - | n.d. | - | n.d. | - |
N-Acetyl Manosamine (ManNAc) | n.d. | - | n.d. | - | n.d. | - |
Heptose | 55.5 | 23.2 | 32.3 | 13.7 | 31.2 | 58.8 |
KDO | 11.8 | 4.3 | 5.9 | 2.8 | 4.8 | 8.0 |
Total | 204.9 | 100.0 | 202.7 | 100.0 | 51.0 | 100.0 |