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. 2017 May 10;3(5):e1602272. doi: 10.1126/sciadv.1602272

Fig. 2. Extracellular recording of spontaneous discharges modulated by ERG inhibitors in acute STN (subthalamic) slices.

Fig. 2

(A to D) Representative cell-attached recordings show that both E-4031 and dofetilide reversibly reduce burst discharges in a dose-dependent fashion (A and B) but do not affect tonic discharges (C and D) (see also fig. S5). (E to H) For the burst mode of discharges, burst rates (average burst counts per minute) (E and F), intraburst spike frequency (G), and the number of spikes per burst (H) before, during, and after application of 5 μM (n = 6) or 10 μM (n = 5) E-4031 are analyzed. *P < 0.05 compared with control, paired two-tailed Student’s t test. N.A., not applicable (no bursts for analysis in that condition). There is no statistically significant difference between “control” and “washout.” *P = 0.13, 0.06, and 0.13 for (F), (G), and (H), respectively, in the study of recovery from 5 μM E-4031 treatment; *P = 0.11, 0.16, and 0.16 for (F), (G), and (H), respectively, in the study of recovery from 10 μM E-4031 treatment. (I to L) For the tonic mode of discharges, single-spike frequency and coefficient of variance (CV) of the interspike intervals (ISIs) before, during, and after E-4031 (I and J; n = 4 in 5 μM and n = 5 in 10 μM) or dofetilide (K and L); n = 3 in 5 μM). Scale bars, 2 s. Animals used: p18 to p26 mice.