Fig. 2. Specificity of paAIP2 inhibition.
A, Effect of paAIP2 under blue-light was tested on different kinases. Phosphorylation of purified Syn-1 peptide by various active kinases was detected by western blotting. PaAIP2 blocks CaMKII activity at various concentrations, while having no effect on other kinases.
B, Quantification of (A). For data analysis, kinase activity of lane 2 was normalized to 100% for each kinase. Error bars indicate S.E.M. for three independent experiments except PAK3 (n=4).
See also Figure S1.