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. Author manuscript; available in PMC: 2018 Apr 5.
Published in final edited form as: Neuron. 2017 Mar 16;94(1):37–47.e5. doi: 10.1016/j.neuron.2017.02.036

Fig. 5. Inhibition of spine enlargement under precise temporal control of CaMKII inhibition with paAIP2.

Fig. 5

A, Averaged time course of spine volume change under different temporal inhibition of CaMKII. During single-spine stimulation with 2-photon glutamate uncaging (0.5 Hz, 30 pulses), neurons were illuminated with blue light for 1 min with different timing relative to the onset of glutamate uncaging (Time shift 0, 10, 30, 60 s). Fluorescence intensity of mEGFP was used to measure the spine volume change. The numbers of samples are indicated in the panels.

B, Transient volume change (volume change at 2 min subtracted by that averaged over 10–22 min) and sustained volume change (volume change averaged over 10–22 min) as a function of time shift. Stars denote statistical significance (p < 0.05, nonparametric one-way ANOVA followed by post-hoc tests using the Tukey’s least significant difference).